| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of Luciferase of Luciferase |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Luciferase Mouse Monoclonal Antibody (6B8) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Luciferase. It has been validated for Immunofluorescence, Western Blot and reacts with the listed species.
Validated Applications
| Target | Luciferase |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6B8 |
| Conjugate | Unconjugated |
| Reactivity | Firefly |
| Applications | Immunofluorescence, Western Blot |
| Purification | Affinity Purified |
| Specificity | Luciferase protein detects endogenous levels of Luciferase |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | P08659 (Human) |
Recommended dilutions: IF 1:50-200;WB 1:1000-2000
Immunogen / Antigen
Recombinant Protein of Luciferase of Luciferase
Scientific Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot. Recommended dilutions: IF 1:50-200;WB 1:1000-2000
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 6B8.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.