| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MAD1L1;MAD1;TXBP181;Mitotic spindle assembly checkpoint protein MAD1;Mitotic arrest deficient 1-like protein 1;MAD1-like protein 1;Mitotic checkpoint MAD1 protein homolog;HsMAD1;hMAD1;Tax-binding protein 181 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428. AA range:394-443 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MAD1 (Phospho Ser428) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MAD1. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | MAD1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-MAD1 (S428) Polyclonal Antibody detects endogenous levels of MAD1 protein only when phosphorylated at S428. |
| Molecular Weight | Calculated: 83 kDa |
| UniProt / Swiss-Prot | Q9Y6D9 (Human) | Q9WTX8 (Mouse) |
Recommended dilutions: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428. AA range:394-443
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
