U-Blot® MAD1 Rabbit Polyclonal Antibody

SKU:BHA21807692
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-MAD1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunofluorescence, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP13049-50 50 uL
WP13049-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MAD1
Antigen
  • Mitotic spindle assembly checkpoint protein MAD1
Alternative names MAD1L1;MAD1;TXBP181;Mitotic spindle assembly checkpoint protein MAD1;Mitotic arrest deficient 1-like protein 1;MAD1-like protein 1;Mitotic checkpoint MAD1 protein homolog;HsMAD1;hMAD1;Tax-binding protein 181
Gene ID 8379 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human MAD1. AA range:394-443
Conjugate
  • Unconjugated
Molecular weight Calculated: 83 kDa
Purification Affinity Purified
Cellular localization Nucleus . Chromosome, centromere, kinetochore . Nucleus envelope . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (PubMed:22351768, PubMed:18981471). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody (PubMed:9546394). Localizes to kinetochores during prometaphase (PubMed:22351768, PubMed:29162720). Does not localize to kinetochores during metaphase (PubMed:29162720). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768). .; [Isoform 3]: Cytoplasm .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP13049
Main SKU BHA21807692
Antibody

Product Overview

U-Blot® MAD1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MAD1. It has been validated for Immunofluorescence, ELISA and reacts with Human, Mouse.

Validated Applications

Target MAD1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Immunofluorescence, ELISA
Purification Affinity Purified
Specificity MAD1 Polyclonal Antibody detects endogenous levels of MAD1 protein.
Molecular Weight Calculated: 83 kDa
UniProt / Swiss-Prot Q9Y6D9 (Human) | Q9WTX8 (Mouse)

Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human MAD1. AA range:394-443

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunofluorescence, ELISA. Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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