| Field | Specification |
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| Antigen | |
| Gene ID | |
| Host | |
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| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Mast cell tryptase AA range: 200-275 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Mast cell tryptase (ABT-MCT) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Mast cell tryptase. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Mast cell tryptase |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-MCT |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody can specifically recognize human Mast cell tryptase protein. |
| Molecular Weight | Calculated: 30 kDa | Observed: 30 kDa |
| UniProt / Swiss-Prot | Q15661 (Human) |
Recommended dilutions: IHC 1:200-400;IF 1:50-200;ELISA 1:500-5000
Immunogen / Antigen
Synthesized peptide derived from human Mast cell tryptase AA range: 200-275
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate gene
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-400;IF 1:50-200;ELISA 1:500-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-MCT.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
