| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MBD3;Methyl-CpG-binding domain protein 3;Methyl-CpG-binding protein MBD3 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MBD3. AA range:221-270 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MBD3 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MBD3. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse.
Validated Applications
| Target | MBD3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | MBD3 Polyclonal Antibody detects endogenous levels of MBD3 protein. |
| Molecular Weight | Observed: 33 kDa |
| UniProt / Swiss-Prot | O95983 (Human) | Q9Z2D8 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human MBD3. AA range:221-270
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
