| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MDM2;E3 ubiquitin-protein ligase Mdm2;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human MDM2. AA range:381-430 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® MDM2 Rabbit Polyclonal Antibody (WP15263) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MDM2. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | MDM2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | MDM2 Polyclonal Antibody detects endogenous levels of MDM2 protein. |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q00987 (Human) | P23804 (Mouse) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human MDM2. AA range:381-430
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
