| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MAP2K2;MEK2;MKK2;PRKMK2;Dual specificity mitogen-activated protein kinase kinase 2;MAP kinase kinase 2;MAPKK 2;ERK activator kinase 2;MAPK/ERK kinase 2;MEK 2 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of human MEK-2 expressed in E. Coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MEK-2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against MEK2. It has been validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | MEK2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | MEK-2 Monoclonal Antibody detects endogenous levels of MEK-2 protein. |
| Molecular Weight | Calculated: 44 kDa |
| UniProt / Swiss-Prot | P36507 (Human) | Q63932 (Mouse) | P36506 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human MEK-2 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
