| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;[Cleaved into: Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form] |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Mesothelin AA range: 400-500 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Mesothelin (ABT-MSLN) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Mesothelin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Mesothelin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-MSLN |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of Mesothelin protein. |
| Molecular Weight | Calculated: 69 kDa | Observed: 65 kDa,45 kDa |
| UniProt / Swiss-Prot | Q13421 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Mesothelin AA range: 400-500
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-MSLN.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
