| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;[Cleaved into: Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form] |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Isotype | |
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| Product Type | |
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| Target |
Product Overview
U-Blot® Mesothelin Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Mesothelin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human.
Validated Applications
| Target | Mesothelin |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1514R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 69 kDa | Observed: 48 kDa(cleaved),70 kDa(precursor) |
| UniProt / Swiss-Prot | Q13421 (Human) | Q61468 (Mouse) | Q9ERA7 (Rat) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:1000-1:20000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
MSLN
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:1000-1:20000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1514R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
