| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MYL12A;MLCB;MRLC3;RLC;Myosin regulatory light chain 12A;MLC-2B;Myosin RLC;Myosin regulatory light chain 2;nonsarcomeric;Myosin regulatory light chain MRLC3;MYL12B;MRLC2;MYLC2B;Myosin regulatory light chain 12B;MLC-2A;MLC-2;M |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MLC-2 (Phospho Thr17/S18) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MLC-2. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | MLC-2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-MLC-2 (T17/S18) Polyclonal Antibody detects endogenous levels of MLC-2 protein only when phosphorylated at T17/S18. |
| Molecular Weight | Calculated: 20 kDa |
| UniProt / Swiss-Prot | P19105;O14950;P24844 (Human) | P18666;Q64122 (Rat) |
Recommended dilutions: IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
