| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Myosin light chain kinase, smooth muscle;MLCK;smMLCK;Kinase-related protein;KRP;Telokin;[Cleaved into: Myosin light chain kinase, smooth muscle, deglutamylated form] |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human MLCK (Phospho Tyr471) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MLCK (Phospho Tyr471) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MLCK. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | MLCK |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat MLCK (Phospho Tyr471) |
| Molecular Weight | Observed: 200 kDa |
| UniProt / Swiss-Prot | Q15746 (Human) | Q6PDN3 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
Synthesized peptide derived from human MLCK (Phospho Tyr471)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
myosin light chain kinase(MYLK) Homo sapiens This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
