| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MLH1/COCA2/DNA mismatch repair protein Mlh1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MLH1 Mouse Monoclonal Antibody [ABT018] is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against MLH1. It has been validated for Western Blot, Immunohistochemistry and reacts with Human.
Validated Applications
| Target | MLH1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT018 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of MLH1 protein. |
| Molecular Weight | Calculated: 85 kDa | Observed: 85 kDa |
| UniProt / Swiss-Prot | P40692 (Human) |
Recommended dilutions: IHC 1:200-1:400; WB 1:2000-1:10000;Note:For IHC,wesuggest antigen retrieval with TE buffer pH 9.0
Immunogen / Antigen
DNA mismatch repair protein Mlh1
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: IHC 1:200-1:400; WB 1:2000-1:10000;Note:For IHC,wesuggest antigen retrieval with TE buffer pH 9.0
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT018.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
