| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Mixed lineage kinase domain-like protein |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human MLKL (phospho S358) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MLKL (Phospho Ser358) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MLKL. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence and reacts with Human.
Validated Applications
| Target | MLKL |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous phospho levels of MLKL (phospho S358) at Human, Mouse, Rat |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q8NB16 (Human) |
Recommended dilutions: IHC 1:100-500;IF/ICC1:100-500
Immunogen / Antigen
Synthesized peptide derived from human MLKL (phospho S358)
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC 1:100-500;IF/ICC1:100-500
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
