| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MLL;ALL1;CXXC7;HRX;HTRX;KMT2A;MLL1;TRX1;Histone-lysine N-methyltransferase MLL;ALL-1;CXXC-type zinc finger protein 7;Lysine N-methyltransferase 2A;KMT2A;Trithorax-like protein;Zinc finger protein HRX |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® MLL Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against MLL. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | MLL |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | MLL Monoclonal Antibody detects endogenous levels of MLL protein. |
| Molecular Weight | Calculated: 432 kDa |
| UniProt / Swiss-Prot | Q03164 (Human) | P55200 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefS
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
