| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Matrix metalloproteinase-9;MMP-9;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;[Cleaved into: 67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9] |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human MMP9 (Cleaved-Met94) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MMP9 (Cleaved-Met94) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MMP-9. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | MMP-9 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human MMP9 (Cleaved-Met94, protein was cleaved amino acid sequence between 93-94 ) |
| Molecular Weight | Observed: 68 kDa,78 kDa |
| UniProt / Swiss-Prot | P14780 (Human) | P41245 (Mouse) | P50282 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
Synthesized peptide derived from human MMP9 (Cleaved-Met94)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:Cleavage of gelatin types I and V and collagen types IV and V.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 3 calcium ions per subunit.,disease:Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Inhibited by histatin-3 1/24 (histatin-5).,function:May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.,induction:Activated by 4-aminophenylmercuric acetate and phorbol ester.,miscellaneous:In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.,PTM:N- and O-glycosylated.,PTM:Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 3 fibronectin type-II domains.,similarity:Contains 4 hemopexin-like domains.,subunit:Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.,tissue specificity:Produced by normal alveolar macrophages and granulocytes.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
