U-Blot® MPP1 Rabbit Polyclonal Antibody (WP13224)

SKU:BHA21807885
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-MPP1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunofluorescence, ELISA, Immunohistochemistry with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Immunofluorescence, ELISA, Immunohistochemistry
Options selector
Catalog no. Size
WP13224-50 50 uL
WP13224-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MPP1
Antigen
  • Kinesin-like protein KIF20B
Alternative names KIF20B;KRMP1;MPHOSPH1;Kinesin-like protein KIF20B;Cancer/testis antigen 90;CT90;Kinesin-related motor interacting with PIN1;M-phase phosphoprotein 1;MPP1
Gene ID 9585 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Immunofluorescence
  • ELISA
  • Immunohistochemistry
Immunogen The antiserum was produced against synthesized peptide derived from human KIF20B. AA range:1771-1820
Conjugate
  • Unconjugated
Molecular weight Observed: 210 kDa
Purification Affinity Purified
Cellular localization Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus, nucleolus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Midbody . Cell projection, axon . Cell projection, growth cone . Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules (PubMed:12740395). Localized to the central spindle during cytokinetic furrowing and with the midbody during abscission (PubMed:12740395, PubMed:17409436). A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase (PubMed:12740395). During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone (PubMed:12740395). At anaphase it is concentrated in the midbody (PubMed:12740395). Colocalized partially along microtubules in primary neurons. Colocalized with SHTN1 along microtubules to the tip of the growing cone in primary hippocampal neurons. Localized in midbodies between dividing radial progenitors in the ventricular zone (By similarity). Colocalized with PRC1 in the nucleus of bladder carcinoma cells at the interphase. Colocalized with PRC1 in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP13224
Main SKU BHA21807885
Antibody

Product Overview

U-Blot® MPP1 Rabbit Polyclonal Antibody (WP13224) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MPP1. It has been validated for Immunofluorescence, ELISA, Immunohistochemistry and reacts with Human, Mouse.

Validated Applications

Target MPP1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Immunofluorescence, ELISA, Immunohistochemistry
Purification Affinity Purified
Specificity MPP1 Polyclonal Antibody detects endogenous levels of MPP1 protein.
Molecular Weight Observed: 210 kDa
UniProt / Swiss-Prot Q96Q89 (Human) | Q80WE4 (Mouse)

Recommended dilutions: IF/ICC1:50-300;ELISA 1:2000-20000;IHC 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human KIF20B. AA range:1771-1820

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

KIF20B (Kinesin Family Member 20B) is a Protein Coding gene. Among its related pathways are Golgi-to-ER retrograde transport and Factors involved in megakaryocyte development and platelet production. GO annotations related to this gene include ATPase activity and microtubule motor activity. An important paralog of this gene is KIF23.

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunofluorescence, ELISA, Immunohistochemistry. Recommended dilutions: IF/ICC1:50-300;ELISA 1:2000-20000;IHC 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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