| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MRPS25;RPMS25;28S ribosomal protein S25;mitochondrial;MRP-S25;S25mt |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® MRP-S25 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against MRP-S25. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | MRP-S25 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | MRP-S25 Polyclonal Antibody detects endogenous levels of MRP-S25 protein. |
| Molecular Weight | Observed: 20 kDa |
| UniProt / Swiss-Prot | P82663 (Human) | Q9D125 (Mouse) | Q4QR80 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
