| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MTOR;FRAP;FRAP1;FRAP2;RAFT1;RAPT1;Serine/threonine-protein kinase mTOR;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic tar |
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| Host | |
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| Clonality | |
| Isotype | |
| Reactivity | |
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Product Overview
U-Blot® mTOR (Phospho Ser2448) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against mTOR. Recognizes Phospho (Ser2448). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | mTOR |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0498R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | This antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448.This antibody does not recognize phosphorylated at other sites. |
| Molecular Weight | Calculated: 289 kDa | Observed: 250 kDa |
| UniProt / Swiss-Prot | P42345 (Human) | Q9JLN9 (Mouse) | P42346 (Rat) |
Recommended dilutions: IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Serine/threonine-protein kinase mTOR
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0498R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
