| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DNA mismatch repair protein Msh6;G/T mismatch binding protein;G/T mismatch-binding protein;GTBP;GTMBP;hMSH6;HNPCC 5;HNPCC5;HSAP;MSH 6;MSH6;MSH6_HUMAN;mutS;E. coli;homolog 6;MutS alpha 160 kDa subunit;MutS homolog 6;E. coli;mutS homolog 6;MutS-alpha 160 kDa subunit;p160;Sperm associated protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human MutS Protein Homolog 6(MSH6) AA range: 900-1000 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® MutS Protein Homolog 6(MSH6) (ABT-MSH6) Mouse Monoclonal Antibody (WM1431) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against MSH6. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | MSH6 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-MSH6 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human MSH6 protein. In western blotting of HEK293 cell lysate, the antibody can label a 180 kDa band corresponding to MSH6, while there is no band detected in MSH6 knock out HEK293 cell lysate. |
| Molecular Weight | Calculated: 153 kDa | Observed: 180 kDa |
| UniProt / Swiss-Prot | P52701 (Human) |
Recommended dilutions: IHC 1:100-500;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human MutS Protein Homolog 6(MSH6) AA range: 900-1000
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
MSH6 is an important gene in the human mismatch repair gene family, which is located at position 16 of the p arm of chromosome 2. Human mismatch repair genes play an important role in maintaining the integrity and stability of genetic information and avoiding genetic mutation. The deletion of this gene after mutation will make the cell mismatch repair function defective, lead to microsatellite instability (MSI), and make the tumor susceptible. Clinically, it is often used in the screening of Lynch syndrome together with mismatch repair proteins MLH1, MSH2 and PMS2.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-500;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-MSH6.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
