U-Blot® MYH9 Rabbit Monoclonal Antibody

SKU:BHA21800911
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UcallM Biosciences Inc
UcallM Biosciences Inc
Details Products
Overview
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Anti-MYH9 antibody (Rabbit host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation with reactivity to Human, Mouse, Rat.
Clonality Monoclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG, Kappa
Clone PT1151R
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Options selector
Catalog no. Size
WR4921-50 50 uL
WR4921-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MYH9
Antigen
  • Myosin-9 (Cellular myosin heavy chain
  • type A) (Myosin heavy chain 9) (Myosin heavy chain
  • non-muscle IIa) (Non-muscle myosin heavy chain A) (NMMHC-A) (Non-muscle myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)
Gene ID 4627 (Human) | 17886 (Mouse) | 25745 (Rat)
Host Rabbit
Clone PT1151R
Clonality
  • Monoclonal
Isotype
  • IgG
  • Kappa
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
Conjugate
  • Unconjugated
Molecular weight Calculated: 227 kDa | Observed: 227 kDa
Purification Protein A
Cellular localization Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Cytoplasmic vesicle, secretory vesicle, Cortical granule . Colocalizes with actin filaments at lamellipodia margins and at the leading edge of migrating cells (PubMed:20052411). In retinal pigment epithelial cells, predominantly localized to stress fiber-like structures with some localization to cytoplasmic puncta (PubMed:27331610). .
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WR4921
Main SKU BHA21800911
Antibody

Product Overview

U-Blot® MYH9 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against MYH9. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target MYH9
Host Rabbit
Clonality Monoclonal
Clone PT1151R
Isotype IgG, Kappa
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Purification Protein A
Specificity Endogenous
Molecular Weight Calculated: 227 kDa | Observed: 227 kDa
UniProt / Swiss-Prot P35579 (Human) | Q8VDD5 (Mouse) | Q62812 (Rat)

Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200

Immunogen / Antigen

Myosin-9 (Cellular myosin heavy chain, type A) (Myosin heavy chain 9) (Myosin heavy chain, non-muscle IIa) (Non-muscle myosin heavy chain A) (NMMHC-A) (Non-muscle myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone PT1151R.

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