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Product Overview
U-Blot® N6-methyladenosine (m6A) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against m6A. It has been validated for Dot Blot and reacts with the listed species.
Validated Applications
| Target | m6A |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0889R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Dot Blot |
| Purification | Protein A |
| Specificity | Endogenous |
Recommended dilutions: DB 1:500-1:2000
Scientific Background
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
Safety & Handling
This antibody is validated for Dot Blot. Recommended dilutions: DB 1:500-1:2000
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0889R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
