| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ATP1A1;Sodium/potassium-transporting ATPase subunit alpha-1;Na;+;/K;+;ATPase alpha-1 subunit;Sodium pump subunit alpha-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Na+/K+-ATPase α1 (Phospho Ser23) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Na+/K+-ATPase α1. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Rat.
Validated Applications
| Target | Na+/K+-ATPase α1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Rat |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at S23. |
| Molecular Weight | Observed: 113 kDa |
| UniProt / Swiss-Prot | P05023 (Human) | P06685 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IF 1:100-1:300;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64
Reactivity Notes
Confirmed cross-reactivity: Rat. Confirm suitability for untested species before use.
Scientific Background
The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:100-1:300;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
