| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NANOGP8;Putative homeobox protein NANOGP8 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Nanog P8 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Nanog P8. It has been validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry and reacts with Human.
Validated Applications
| Target | Nanog P8 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Nanog P8 Polyclonal Antibody detects endogenous levels of Nanog P8 protein. |
| Molecular Weight | Observed: 35 kDa |
| UniProt / Swiss-Prot | Q6NSW7 (Human) |
Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
