| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NCOA3;AIB1;BHLHE42;RAC3;TRAM1;Nuclear receptor coactivator 3;NCoA-3;ACTR;Amplified in breast cancer 1 protein;AIB-1;CBP-interacting protein;pCIP;Class E basic helix-loop-helix protein 42;bHLHe42;Receptor-associated coactivator 3;RAC-3;Steroid receptor coactivator protein 3;SRC-3;Thyroid hormone receptor activator molecule 1;TRAM-1 |
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| Cellular Localization | |
| Clonality | |
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| Gene ID | |
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Product Overview
U-Blot® NCoA 3 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against NCoA-3. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NCoA-3 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1635R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 155 kDa | Observed: 160 kDa |
| UniProt / Swiss-Prot | Q9Y6Q9 (Human) | O09000 (Mouse) | Q9EPU2 (Rat) |
Recommended dilutions: IHC 1:200-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Nuclear receptor coactivator 3
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1635R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
