| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1;Complex I-MWFE;CI-MWFE;NADH-ubiquinone oxidoreductase MWFE subunit |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human NDUFA1 AA range: 20-100 |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® NDUFA1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NDUFA1. It has been validated for Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NDUFA1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of human NDUFA1 |
| UniProt / Swiss-Prot | O15239 (Human) |
Recommended dilutions: IHC 1:50-200;ELISA(peptide)1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from human NDUFA1 AA range: 20-100
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, ELISA. Recommended dilutions: IHC 1:50-200;ELISA(peptide)1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
