| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Neurofilament AA range: 400-543 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Neurofilament (ABT456) Mouse Monoclonal Antibody (WM1503) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against NF-L. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NF-L |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT456 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Neurofilament protein, especilaly NF-L protein. |
| Molecular Weight | Calculated: 68 kDa | Observed: 68 kDa |
| UniProt / Swiss-Prot | P07196 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Neurofilament AA range: 400-543
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Neurofilament (NF) is a neuron specific intermediate filament protein. It is a polymer composed of subunits of NF-H, NF-M and NF-L. It exists in neurons, neurites, peripheral nerve fibers, sympathetic ganglion cells and adrenal medulla. It is expressed in ganglioneuroma, paraganglioma, cerebellar or peripheral neuroblastoma, adrenal tumor or peripheral pheochromocytoma. It is helpful to the diagnosis and differential diagnosis of neuroblastoma and pheochromocytoma, and to identify the neurons of Hirschsprung's disease.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT456.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
