| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NFKB2;LYT10;Nuclear factor NF-kappa-B p100 subunit;DNA-binding factor KBF2;H2TF1;Lymphocyte translocation chromosome 10 protein;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;Oncogene Lyt-10;Lyt10 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52 around the phosphorylation site of Ser865. AA range:833-882 |
| Isotype | |
| Molecular Weight | |
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| Reactivity | |
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| Target |
Product Overview
U-Blot® NFκB-p100 (Phospho Ser865) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NF-κB p100/p52. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NF-κB p100/p52 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-NFκB-p100 (S865) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S865. |
| Molecular Weight | Calculated: 97 kDa |
| UniProt / Swiss-Prot | Q00653 (Human) | Q9WTK5 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52 around the phosphorylation site of Ser865. AA range:833-882
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
