| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;[Cleaved into: Nuclear factor NF-kappa-B p50 subunit] |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human NF κB P105/P50 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® NF κB P105/P50 Mouse Monoclonal Antibody (4D11) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against NFKB1. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse.
Validated Applications
| Target | NFKB1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D11 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of NF κB P105/P50 at Human, Mouse |
| Molecular Weight | Observed: 50 kDa,100 kDa |
| UniProt / Swiss-Prot | P19838 (Human) | P25799 (Mouse) | Q63369 (Rat) |
Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Immunogen / Antigen
Synthesized peptide derived from human NF κB P105/P50
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4D11.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
