U-Blot® NFAT1 (Phospho Ser326) Rabbit Polyclonal Antibody (WP10554)

SKU:BHA21804949
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-NFAT1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA, Immunohistochemistry with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA, Immunohistochemistry
Options selector
Catalog no. Size
WP10554-50 50 uL
WP10554-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NFAT1
Antigen
  • NFAT1 (Phospho Ser326)
Alternative names Nuclear factor of activated T-cells, cytoplasmic 2;NF-ATc2;NFATc2;NFAT pre-existing subunit;NF-ATp;T-cell transcription factor NFAT1
Gene ID 4773 (Human) | 18019 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
  • Immunohistochemistry
Immunogen Synthesized peptide derived from human NFAT1 (Phospho Ser326)
Conjugate
  • Unconjugated
Molecular weight Observed: 100 kDa
Purification Affinity Purified
Cellular localization Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10554
Main SKU BHA21804949
Antibody

Product Overview

U-Blot® NFAT1 (Phospho Ser326) Rabbit Polyclonal Antibody (WP10554) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NFAT1. Recognizes Phospho. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.

Validated Applications

Target NFAT1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA, Immunohistochemistry
Purification Affinity Purified
Specificity This antibody detects endogenous levels of Human,Mouse,Rat NFAT1 (Phospho Ser326)
Molecular Weight Observed: 100 kDa
UniProt / Swiss-Prot Q13469 (Human) | Q60591 (Mouse)

Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

Immunogen / Antigen

Synthesized peptide derived from human NFAT1 (Phospho Ser326)

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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