U-Blot® NHERF-2 Rabbit Polyclonal Antibody

SKU:BHA21810574
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-NHERF-2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP15669-50 50 uL
WP15669-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NHERF-2
Antigen
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Alternative names SLC9A3R2;NHERF2;Na;+;/H;+;exchange regulatory cofactor NHE-RF2;NHERF-2;NHE3 kinase A regulatory protein E3KARP;SRY-interacting protein 1;SIP-1;Sodium-hydrogen exchanger regulatory factor 2;Solute carrier family 9 isoform A3 regulatory factor 2;Tyrosine kinase activator protein 1;TKA-1
Gene ID 9351 (Human) | 65962 (Mouse) | 116501 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from NHERF-2 . at AA range: 20-100
Conjugate
  • Unconjugated
Molecular weight Observed: 37 kDa
Purification Affinity Purified
Cellular localization Endomembrane system ; Peripheral membrane protein . Nucleus . Apical cell membrane . Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP15669
Main SKU BHA21810574
Antibody

Product Overview

U-Blot® NHERF-2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NHERF-2. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target NHERF-2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity NHERF-2 Polyclonal Antibody detects endogenous levels of NHERF-2 protein.
Molecular Weight Observed: 37 kDa
UniProt / Swiss-Prot Q15599 (Human) | Q9JHL1 (Mouse) | Q920G2 (Rat)

Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.

Immunogen / Antigen

Synthesized peptide derived from NHERF-2 . at AA range: 20-100

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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