| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ZC3HC1;NIPA;HSPC216;Nuclear-interacting partner of ALK;Nuclear-interacting partner of anaplastic lymphoma kinase;hNIPA;Zinc finger C3HC-type protein 1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NIPA around the phosphorylation site of Ser354. AA range:320-369 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® NIPA (Phospho Ser354) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NIPA. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | NIPA |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-NIPA (S354) Polyclonal Antibody detects endogenous levels of NIPA protein only when phosphorylated at S354. |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q86WB0 (Human) | Q80YV2 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human NIPA around the phosphorylation site of Ser354. AA range:320-369
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
