| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NME1;NDPKA;NM23;Nucleoside diphosphate kinase A;NDK A;NDP kinase A;Granzyme A-activated DNase;GAAD;Metastasis inhibition factor nm23;Tumor metastatic process-associated protein;nm23-H1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human NM23-H1 expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® NM23-H1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against NM23-H1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human.
Validated Applications
| Target | NM23-H1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | NM23-H1 Monoclonal Antibody detects endogenous levels of NM23-H1 protein. |
| Molecular Weight | Calculated: 17 kDa |
| UniProt / Swiss-Prot | P15531 (Human) | P15532 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human NM23-H1 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
