U-Blot® NM23-H2 Rabbit Polyclonal Antibody

SKU:BHA21808183
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-NM23-H2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP13495-50 50 uL
WP13495-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NM23-H2
Antigen
  • Nucleoside diphosphate kinase B
Alternative names NME2;NM23B;Nucleoside diphosphate kinase B;NDK B;NDP kinase B;C-myc purine-binding transcription factor PUF;Histidine protein kinase NDKB;nm23-H2
Gene ID 4831;654364 (Human) | 18103 (Mouse) | 83782 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human NM23. AA range:91-140
Conjugate
  • Unconjugated
Molecular weight Observed: 23 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Cell projection, lamellipodium . Cell projection, ruffle . Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates. .; [Isoform 1]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus .; [Isoform 3]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP13495
Main SKU BHA21808183
Antibody

Product Overview

U-Blot® NM23-H2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NM23-H2. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target NM23-H2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity NM23-H2 Polyclonal Antibody detects endogenous levels of NM23-H2 protein.
Molecular Weight Observed: 23 kDa
UniProt / Swiss-Prot P22392 (Human) | Q01768 (Mouse) | P19804 (Rat)

Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human NM23. AA range:91-140

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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