| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CYBB;NOX2;Cytochrome b-245 heavy chain;CGD91-phox;Cytochrome b;558;subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome |
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Product Overview
U-Blot® NOX2 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against gp91-phox. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | gp91-phox |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0125R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 65 kDa | Observed: 65 kDa |
| UniProt / Swiss-Prot | P04839 (Human) | Q61093 (Mouse) |
Recommended dilutions: WB 1:1000-5000;ELISA 1:5000-20000
Immunogen / Antigen
Cytochrome b-245 heavy chain
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-5000;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0125R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
