| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 2 phospho D glyceRate hydrolyase;2-phospho-D-glyceRate hydro-lyase;Eno 2;ENO2;ENOG;ENOG_HUMAN;Enolase 2;gamma, neuronal;Enolase 2;Enolase 2 gamma neuronal;Enolase2;Epididymis secretory protein Li 279;Gamma enolase;Gamma-enolase;HEL S 279;Neural enolase;Neuron specific enolase;Neuron specific gamma enolase;Neuron-specific enolase;neuronal enriched enolase;Neurone specific enolase;NSE |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Neuron-Specific Enolase(NSE) AA range: 300-434 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® NSE (PT2281) Mouse Monoclonal Antibody (WM1437) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Enolase. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Enolase |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PT2281 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human NSE protein. In western blotting of PC-12 cell lysate, the antibody can label a 47 kDa band corresponding to NSE. |
| Molecular Weight | Calculated: 47 kDa | Observed: 47 kDa |
| UniProt / Swiss-Prot | P09104 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Neuron-Specific Enolase(NSE) AA range: 300-434
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Neuron-specific enolase (NSE) has α、β、γ three subunits, mainly distributed in central nerve, peripheral nerve, neuroendocrine cells and tumor cells, can be used in the auxiliary diagnosis of neuroendocrine tumors. Some non neuroendocrine cells also express NSE, such as normal smooth muscle, myoepithelial cells, renal tubular cells, lymphocytes and so on. It should be combined with other antibodies in the study of neuroendocrine tumors.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT2281.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
