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Product Overview
U-Blot® Nucleolin Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against NUCL. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | NUCL |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0100R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 77 kDa | Observed: 110 kDa |
| UniProt / Swiss-Prot | P19338 (Human) | P09405 (Mouse) | P13383 (Rat) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Nucleolin (Protein C23)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0100R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
