| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nucleolar protein NO38;Numatrin |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Nucleophosmin AA range: 100-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Nucleophosmin (ABT486) Mouse Monoclonal Antibody (WM1504) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Nucleophosmin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Nucleophosmin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT486 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Nucleophosmin protein. |
| Molecular Weight | Calculated: 32 kDa | Observed: 33 kDa |
| UniProt / Swiss-Prot | P06748 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Nucleophosmin AA range: 100-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Nucleophosmin is the most abundant nuclear phosphoprotein in cells, which usually accumulates in the cytoplasm. Generally speaking, the content of tumor growing cells is higher than that of normal quiescent cells. It can be used as a nuclear marker, as well as for the observation of the efficacy of anticancer drugs and the study of cell apoptosis.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT486.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
