U-Blot® NuMA Rabbit Polyclonal Antibody

SKU:BHA21808241
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    Overview
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    Anti-NuMA antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
    Clonality Polyclonal
    Host Rabbit
    Reactivity Human, Mouse
    Conjugate Unconjugated
    Isotype IgG
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
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    Catalog no. Size
    WP13548-50 50 uL
    WP13548-100 100 uL
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size: 50 uL
    • Lead time: options listed in "Availability Content"; other statuses may take longer.
    • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Field Specification
    Alternative Names NUMA1;NUMA;Nuclear mitotic apparatus protein 1;NuMA protein;SP-H antigen
    Antigen
    • Nuclear mitotic apparatus protein 1
    Cellular Localization Nucleus . Nucleus, nucleoplasm . Nucleus matrix . Chromosome . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cell cortex . Cell membrane ; Lipid-anchor ; Cytoplasmic side . Lateral cell membrane . Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826). The localization to the spindle poles is regulated by AAAS (PubMed:26246606). In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553). In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826). In prometaphase, after nuclear envelope disassembly, forms aggregates both in the spindle midzone and at duplicated centrosomes and astral microtubules (MTs) of the bipolar spindle apparatus (PubMed:10811826). Translocates from the spindle midzone towards the spindle poles along spindle fibers in a MT- and dynein-dynactin-dependent manner until the anaphase onset (PubMed:1541636, PubMed:10811826). In metaphase, recruited to the polar cortical region in a GPSM2- and GNAI1-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901). Excluded from the metaphase equatorial cortical region in a RanGTP-dependent manner (PubMed:22327364, PubMed:23870127). Phosphorylation on Thr-2055 by CDK1 results in its localization at spindle poles in metaphase, but not at the cell cortex (PubMed:23921553). In anaphase, recruited and anchored at the cell membrane of the polar cortical region in a EPB41-, EPB41L2-, phosphatidylinositol-dependent and GPSM2- and G(i) alpha proteins-independent manner (PubMed:23870127, PubMed:24996901, PubMed:24109598, PubMed:24371089). Excluded from the anaphase equatorial region of the cell cortex in a RACGAP1- and KIF23-dependent and RanGTP-independent manner (PubMed:24996901). Associated with astral MTs emanating from the spindle poles during anaphase (PubMed:12445386, PubMed:24996901). Nonphosphorylated Thr-2055 localizes at the cell cortex, weakly during metaphase and more prominently during anaphase in a phosphatase PPP2CA-dependent manner (PubMed:23921553). As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident (PubMed:1541636). Localizes to the tips of cortical MTs in prometaphase (PubMed:26765568). Localizes along MTs and specifically to both MT plus and minus ends (PubMed:26765568). Accumulates also at MT tips near the cell periphery (PubMed:26765568). Colocalizes with GPSM2 at mitotic spindle poles during mitosis (PubMed:11781568, PubMed:21816348). Colocalizes with SPAG5 at mitotic spindle at prometaphase and at mitotic spindle poles at metaphase and anaphase (PubMed:27462074). Colocalizes with ABRO1 at mitotic spindle poles (PubMed:26195665). Colocalized with TNKS from prophase through to anaphase in mitosis (PubMed:16076287). Colocalizes with tubulin alpha (PubMed:12445386). CCSAP is essential for its centrosomal localization (PubMed:26562023). In horizontally retinal progenitor dividing cells, localized to the lateral cortical region (By similarity). .; [Isoform 3]: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles. .; [Isoform 4]: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles. .
    Clonality
    • Polyclonal
    Concentration 1 mg/ml
    Conjugate
    • Unconjugated
    Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene ID 4926 (Human)
    Host Rabbit
    Immunogen The antiserum was produced against synthesized peptide derived from human NUMA1. AA range:334-383
    Isotype
    • IgG
    Molecular Weight Observed: 240 kDa
    Product Type
    • Polyclonal Antibody
    • Primary Antibodies
    Reactivity
    • Human
    • Mouse
    Storage -15°C to -25°C/1 year(Do not lower than -25°C)
    Target NuMA
    Antibody

    Product Overview

    U-Blot® NuMA Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NuMA. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

    Validated Applications

    Target NuMA
    Host Rabbit
    Clonality Polyclonal
    Isotype IgG
    Conjugate Unconjugated
    Reactivity Human, Mouse
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
    Purification Affinity Purified
    Specificity NuMA Polyclonal Antibody detects endogenous levels of NuMA protein.
    Molecular Weight Observed: 240 kDa
    UniProt / Swiss-Prot Q14980 (Human)

    Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

    Immunogen / Antigen

    The antiserum was produced against synthesized peptide derived from human NUMA1. AA range:334-383

    Reactivity Notes

    Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

    Scientific Background

    This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

    Safety & Handling

    For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
    What applications is this antibody validated for?

    This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

    What species does it react with?

    Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

    Is this antibody conjugated?

    Conjugate: Unconjugated.

    How should it be stored?

    Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

    Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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