| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | POU5F1;OCT3;OCT4;OTF3;POU domain;class 5, transcription factor 1;Octamer-binding protein 3;Oct-3;Octamer-binding protein 4;Oct-4;Octamer-binding transcription factor 3;OTF-3 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human Oct-3/4 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Oct-3/4 Mouse Monoclonal Antibody (WM0513) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Oct-3/4. It has been validated for Western Blot, Flow Cytometry, Immunofluorescence and reacts with Human.
Validated Applications
| Target | Oct-3/4 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Flow Cytometry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | Oct-3/4 Monoclonal Antibody detects endogenous levels of Oct-3/4 protein. |
| Molecular Weight | Calculated: 39 kDa |
| UniProt / Swiss-Prot | Q01860 (Human) | P20263 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;Flow Cyt 1:100-1:200;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human Oct-3/4 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Safety & Handling
This antibody is validated for Western Blot, Flow Cytometry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;Flow Cyt 1:100-1:200;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
