| Field | Specification |
|---|---|
| Alternative Names | POU5F1;OCT3;OCT4;OTF3;POU domain;class 5, transcription factor 1;Octamer-binding protein 3;Oct-3;Octamer-binding protein 4;Oct-4;Octamer-binding transcription factor 3;OTF-3 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Oct-4 AA range:50-150 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Oct-4 (PTR1369) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against OCT-4. It has been validated for Western Blot, ELISA and reacts with Human.
Validated Applications
| Target | OCT-4 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR1369 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of 4-Oct protein. |
| Molecular Weight | Calculated: 39 kDa | Observed: 40 kDa |
| UniProt / Swiss-Prot | Q01860 (Human) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:1000-5000.
Immunogen / Antigen
Synthesized peptide derived from human Oct-4 AA range:50-150
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:1000-5000.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR1369.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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