| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CDKN1A;CAP20;CDKN1;CIP1;MDA6;PIC1;SDI1;WAF1;Cyclin-dependent kinase inhibitor 1;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® p21 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against p21. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | p21 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | p21 Polyclonal Antibody detects endogenous levels of p21 protein. |
| Molecular Weight | Observed: 21 kDa |
| UniProt / Swiss-Prot | P38936 (Human) | P39689 (Mouse) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
