| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | EP300;P300;Histone acetyltransferase p300;p300 HAT;E1A-associated protein p300 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human p300 AA range: 1-100 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® p300 (PTR2539) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against p300. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | p300 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR2539 |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of p300 protein. |
| Molecular Weight | Calculated: 264 kDa | Observed: 264 kDa |
| UniProt / Swiss-Prot | Q09472 (Human) | B2RWS6 (Mouse) |
Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human p300 AA range: 1-100
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR2539.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
