| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MAPK14;CSBP;CSBP1;CSBP2;CSPB1;MXI2;SAPK2A;Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® p38 (Phospho Thr180/Y182) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against p38. Recognizes Phospho. It has been validated for Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat, Guinea Pig.
Validated Applications
| Target | p38 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Guinea Pig |
| Applications | Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182. |
| Molecular Weight | Observed: 38 kDa |
| UniProt / Swiss-Prot | Q16539 (Human) | P47811 (Mouse) | P70618 (Rat) |
Recommended dilutions: IF/ICC1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC 1:100-500;ELISA 1:5000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Guinea Pig. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry, ELISA. Recommended dilutions: IF/ICC1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC 1:100-500;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat, Guinea Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
