| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | NCF4;SH3PXD4;Neutrophil cytosol factor 4;NCF-4;Neutrophil NADPH oxidase factor 4;SH3 and PX domain-containing protein 4;p40-phox;p40phox |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® p40-phox (Phospho Thr154) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against p40-phox. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | p40-phox |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154. |
| Molecular Weight | Calculated: 39 kDa |
| UniProt / Swiss-Prot | Q15080 (Human) | P97369 (Mouse) |
Recommended dilutions: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
