| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53_HUMAN;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;tumor antigen p55;Tumor protein 53;Tumor protein p53;Tumor suppressor p53 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human protein. AA range:250-393 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® p53 (ABT002) Mouse Monoclonal Antibody (WM1454) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against p53. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | p53 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT002 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can recognize human wild type and mutant P53 protein. In western blotting of wild type HEK293 cell lysate, the antibody can label a 53 kDa band corresponding to P53, while there is no band detected in P53 knock out HEK293 cell lysate. |
| Molecular Weight | Calculated: 53 kDa | Observed: 53 kDa |
| UniProt / Swiss-Prot | P04637 (Human) | P02340 (Mouse) | P10361 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human protein. AA range:250-393
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
P53 is a tumor suppressor gene located on chromosome 17. The mutation of p53 gene will change its spatial conformation and turn into oncogene, and then lose the regulation of cell growth, apoptosis and DNA repair. The mutation or deletion of p53 gene is the cause of many tumors. Compared with the wild-type protein, the mutant p53 protein has a longer half-life, so it is easier to detect by immunohistochemistry. The p53 mutant protein is expressed in many kinds of tumors, such as breast cancer, gastrointestinal cancer, hepatocellular carcinoma and respiratory tract tumor. It is mainly used for the study of various tumors and can be used as an indicator of tumor prognosis.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT002.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
