| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | TP53;P53;Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human p53(Phospho-Ser366) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® p53 (Phospho Ser366) Rabbit Polyclonal Antibody (WP10240) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against p53. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | p53 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous p53(Phospho-Ser366) |
| Molecular Weight | Observed: 53 kDa |
| UniProt / Swiss-Prot | P04637 (Human) | P02340 (Mouse) |
Recommended dilutions: WB 1:500-2000;ELISA 1:10000-20000
Immunogen / Antigen
Synthesized phospho-peptide around the phosphorylation site of human p53(Phospho-Ser366)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;ELISA 1:10000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
