| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PSME3;Proteasome activator complex subunit 3;11S regulator complex subunit gamma;REG-gamma;Activator of multicatalytic protease subunit 3;Ki nuclear autoantigen;Proteasome activator 28 subunit gamma;PA28g;PA28gamma |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PA28γ protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PA28γ Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PA28γ. It has been validated for Western Blot, Immunofluorescence and reacts with Human, Mouse, Dog, Pig.
Validated Applications
| Target | PA28γ |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Dog, Pig |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | PA28γ Monoclonal Antibody detects endogenous levels of PA28γ protein. |
| Molecular Weight | Calculated: 30 kDa |
| UniProt / Swiss-Prot | P61289 (Human) | P61290 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human PA28γ protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Dog, Pig. Confirm suitability for untested species before use.
Scientific Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) o
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Dog, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
