| Field | Specification |
|---|---|
| Alternative Names | 3NT |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Pan-3-Nitrotyrosine Mouse mAb is a monoclonal antibody raised in Mouse that specifically recognizes 3-Nitrotyrosine (Tyrosine nitration [Tyr(Y)]). It is designed for detection and analysis of the Tyrosine nitration [Tyr(Y)] post-translational modification across research applications.
3-Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine. Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body's primary endogenous antioxidants.
Validated Applications
| Application | Western Blot, Immunofluorescence |
|---|---|
| Recommended Dilution | WB 1:500–1:1000, ICC/IF 1:100 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Protein G-conjugated agarose followed by nitrotyrosine peptides agarose affinity chromatography |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse, Rat. It targets the Tyrosine nitration [Tyr(Y)] modification and is suitable for Western Blot, Immunofluorescence.
Safety & Handling
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Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:500–1:1000, ICC/IF 1:100.
Use WB 1:500–1:1000, ICC/IF 1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing 3-Nitrotyrosine (Tyrosine nitration [Tyr(Y)]); reactivity: Human, Mouse, Rat.
The monoclonal antibody (Mouse host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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