| Field | Specification |
|---|---|
| Alternative Names | Kme2 |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Pan-Di-Methyllysine Rabbit mAb-BSA and Azide free is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Di-Methyllysine (Lysine di-methylation [ε-amino group (Nε) of the lysine (Lys, K) side chain]). It is designed for detection and analysis of the Lysine di-methylation [ε-amino group (Nε) of the lysine (Lys, K) side chain] post-translational modification across research applications.
Di-Methyllysine (Kme2) is a crucial reversible post-translational modification of proteins, which occurs on the ε-amino group of lysine residues, including symmetric and asymmetric dimethylation forms. It is widely distributed in histones and non-histone proteins, and participates in regulating chromatin remodeling, gene transcription, RNA splicing, DNA damage repair, cell cycle progression and signal transduction. Aberrant lysine dimethylation is closely associated with tumorigenesis, inflammation, neurodegeneration and metabolic disorders, serving as a vital biomarker for epigenetic and pathological research.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:1000 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse, Rat. It targets the Lysine di-methylation [ε-amino group (Nε) of the lysine (Lys, K) side chain] modification and is suitable for Western Blot.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot. Recommended dilutions: WB 1:1000.
Use WB 1:1000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Di-Methyllysine (Lysine di-methylation [ε-amino group (Nε) of the lysine (Lys, K) side chain]); reactivity: Human, Mouse, Rat.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Upon delivery centrifugate. Reconstitute with 100 uL distilled water (1.0 mg/mL after restoration). Aliquot and store at -80°C. Avoid freeze / thaw cycles. | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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