| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | polypeptide |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Pan-Ubiquitin Rabbit mAb (Clone W-R12) is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Ubiquitin (Ubiquitination [Lys(K)]). It is designed for detection and analysis of the Ubiquitination [Lys(K)] post-translational modification across research applications.
Ubiquitin conjugates to lysine (Lys, K) residues of a broad spectrum of cellular substrate proteins, including histones, cell cycle regulators, transcription factors, signaling molecules, apoptotic proteins, misfolded and damaged proteins. It also forms polyubiquitin chains via internal lysine sites (K6, K11, K27, K29, K33, K48, K63) to mediate protein degradation, DNA repair, gene transcription, cell cycle progression, apoptosis and immune signaling.
Validated Applications
| Application | Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry |
|---|---|
| Recommended Dilution | WB 1:1000, IHC-P 1:2000, ICC 1:500, ICFCM 1:500 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Species Independent |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Species Independent. It targets the Ubiquitination [Lys(K)] modification and is suitable for Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry. Recommended dilutions: WB 1:1000, IHC-P 1:2000, ICC 1:500, ICFCM 1:500.
Use WB 1:1000, IHC-P 1:2000, ICC 1:500, ICFCM 1:500. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Ubiquitin (Ubiquitination [Lys(K)]); reactivity: Species Independent.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
